Tumor-targeting glycol chitosan nanoparticles for in vivo NIR fluorescent imaging
- Tumor-targeting glycol chitosan nanoparticles for in vivo NIR fluorescent imaging
- 나진희; 구희범; 최귀원; 박재형; 권익찬; 정서영; 김광명
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- Objectives: Hydrophobically modified glycol chitosan nanoparticles (CNPs) exhibited enhancedbiocompatibility and tumor-targeting ability in various cancer model.Methods: We prepared hydrophobically modified glycol chitosan nanoparticles by combination of
glycol chitosan and 5b-cho1anic acid and they were labeled with the NIRF dye, Cy5.5. Thesenanoparticles were applied for near infrared fluorescence (NIRF) imaging in mouse tumor models. Tomake liver cancer model, CT26 tumor cells were directly injected into left lobe ofthe liver. In case ofbrain cancer model, the mice were located in a rodent stereotactic frame, and U87 tumor cells wereinjected to a burr hole on the skull, In both models, Cy5.5 labeled CNPs were injected intravenously,and the biodistributions ofthem were evaluated using the eXplore Optix system.Results and Conclusion: These CNPs are unique for their distinctive characteristics, such as
stability in serum, deformability, and rapid uptake by tumor cells compared with other nanoparticles.And these properties enables tumor tissue specific targeting in tumor bearing mice. In liver cancermodel, we showed distinct difference of NIR intensity between the tumor tissues and surroundingnormal liver tissues with nanoparticle. In brain cancer, the images showed the possibility of partialdisruption of blood-brain barrier (BBB) along tumor growth and penetration of nanoparticles.Therefbre, these glycol chitosan nanoparticles hold great promise in simultaneous diagnosis ofdiseaseand monitoring oftreatment.
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