Tumor targeting siRNA delivery system
- Tumor targeting siRNA delivery system
- siRNA; siRNA delivery system; poly-siRNA
- Issue Date
- Gene expression control by the small interfering RNA (siRNA) is a most promising tool for gene therapy by sequence specific cleavage of mRNA. But siRNA’s instability and poor cell penetrating properties are being obstacles for its application. So, it requires effective siRNA delivery system for diverse use. Here we present the new carrier which composed of biocompatible/biodegradable glycol chitosan based nanoparticles (NP) for complex formation with siRNA. Using the Red Fluorescence Protein (RFP) over-expressed mouse melanoma B16F10 cells and athymic nude mouse, delivery effects of NP-siRNA were examined such as fluorescence imaging methods for cellular uptake, gene silencing efficacy and biodistribution etc. Although the development of various gene delivery systems has increased the siRNA delivery efficiency, still many problems remain to be resolved before the clinical application of siRNA. So, we suggest reducible polymerized siRNA a possible solution for low delivery efficiency of siRNA. By increasing the size of siRNA, poly-siRNA formed more condensed and nano-sized complexes due to strong electrostatic interaction, we obtained enhanced cellular uptake of siRNA and powerful gene silencing effect. Our results demonstrate that strong polyanionic, reducible poly-siRNA can serve as a powerful therapeutic agent for treating human diseases.
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