Microbial evaluation of sand- and micro-filtration systems as pretreatment for seawater reverse osmosis desalination using culture-independent analysis based on the 16S rRNA gene
- Microbial evaluation of sand- and micro-filtration systems as pretreatment for seawater reverse osmosis desalination using culture-independent analysis based on the 16S rRNA gene
- 배효관; 정성필; 김희선; 이석헌
- Issue Date
- EuroMed 2010
- , 17-17
- The pilot-plant to desalinate seawater using reverse-osmosis (RO) membrane has been operated for 1.75 years. The seawater from a harbor in southern Korea was treated in the volume of 200 m3 and 100 m3 of desalinated water was produced a day There were two different pretreatment processes of sand- (SF) and micro-filtration (MF) in front of the RO membrane process. The main purpose of operating pretreatment systems for seawater desalination was to prevent fouling on the RO membrane by reducing silt contents, bio-foulants, and sources inducing crystalization. To enhance the removal efficiency of bio-foulants, the disinfection using chlorine (Cl2) was conducted just after the intake of raw seawater. The turbidity of permeate from SF and MF systems were only 0.23 and 0.07, respectively. The physical pretreatments indicated the significant removal of particles including bio-foulants. In addition to the evaluation of SF and MF by the physical property, pretreatment systems should be evaluated through the analysis of the microbial community structure to insure the removal of bio-foulants which are marine biofilm-forming bacteria. In this research, the microbial community structures of the raw seawater and permeates from SF and MF were analyzed focused on the relative abundant of putative biofilm-forming bacteria, Alteromonas macleodii by clone library of the 16S rRNA gene. The selective removal of biofilm-forming bacteria by SF and MF was observed. Alteromonas macleodii was the most abundant bacteria in the raw seawater (28.9%). However, no Alteromonas sp. was identified in the permeate from SF. In the permeate from MF, the relative abundance of Alteromonas macleodii was 6.8 %. The detailed descnption of microbial community structures of raw seawater and permeates from SF and MF will be presented.
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