Channel-mediated tonic GABA release from glia

Channel-mediated tonic GABA release from glia
윤보은이수정Ken berglund오수진박형주신희섭조지어거스틴이창준
tonic GABA; Glia; Bestrophin
Issue Date
Neuroscience 2010
Synaptic inhibition is based on both tonic and phasic release of the inhibitory transmitter, GABA. While phasic GABA release arises from conventional, Ca2+- dependent exocytosis from neurons, the mechanism and source of tonic GABA release are unclear. Here we report that tonic inhibition in the cerebellum is due to GABA being released from glial cells by permeation through the Bestrophin 1 (Best1) anion channel. By developing and utilizing a two-cell biosensor technique, we demonstrate that GABA can directly permeate through Best1 to yield GABA release. Tonic inhibition is eliminated by various drugs that block Best1 and by selective RNA-based silencing of Best1 expression. We confirmed that glial cells express both GABA and Best1 by immunohistochemistry. We found that selective expression of Best1 in glial cells, after preventing general expression of Best1, fully rescues tonic inhibition. Therefore, we conclude that Best1-mediated GABA release from glial cells is responsible for tonic inhibition in the cerebellum. Our results identify a molecular mechanism for tonic inhibition and establish a novel role for neuron-glial interactions in regulating brain function.
Appears in Collections:
KIST Publication > Conference Paper
Files in This Item:
There are no files associated with this item.
RIS (EndNote)
XLS (Excel)


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.