Isolation of Tributyltin Chloride Resistance Bacteria and Rapid Electrochemical Determination of Bacterial Organotin Degradation Activity

Title
Isolation of Tributyltin Chloride Resistance Bacteria and Rapid Electrochemical Determination of Bacterial Organotin Degradation Activity
Authors
정봉근홍석원최용수Rangarajulu Senthil김미아김성현김형주
Keywords
Tributyltin; Electrochemical TBT analysis; TBT resistance bacteria; TBT
Issue Date
2011-01
Publisher
Bulletin of the Korean Chemical Society
Citation
VOL 32, NO 1, 356-358
Abstract
In this study, we isolated the TBT resistance bacterial strain, and the TBT degradation activity was evaluated via an electrochemical method. The most troublesome issue pertinent to environmental hormone studies involving TBT is the lack of an effective and rapid analysis method. Therefore, the application of ASV to the TBT analysis may prove to be both simpler and more rapid than GC-FPD. However, it should also be noted that the TBT analytical method employed herein only provides the total tin concentration in a filtered bacteria-free culture medium, principally as the result of the unpredictable complexation of bacterial debris with tin and tropolone, which induces the prompt contamination of electrodes (result not shown). This, therefore, suggests that our electrochemical method might prove effective in distinguishing between TBT-degrading bacteria and non-degrading bacteria, a conclusion supported by the results regarding TBT degradation during the cultivation of the TBD-1 strain. Further studies are warranted to establish both the physiological characteristics of the bacterial strains and the efficacy of the electrochemical TBT analysis method under varying experimental conditions, including differing preconcentration conditions with different scanning ranges, using different electrode materials.
URI
http://pubs.kist.re.kr/handle/201004/39352
ISSN
0253-2964
Appears in Collections:
KIST Publication > Article
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