Thermal gelling polyalanine-poloxamine-polyalanine aqueous solution for chondrocytes 3D culture: Initial concentration effect

Title
Thermal gelling polyalanine-poloxamine-polyalanine aqueous solution for chondrocytes 3D culture: Initial concentration effect
Authors
최보규Min Hee Park조소혜Min Kyung JooHye Jin OhEun Hye Kim박귀덕한동근정병문
Keywords
Thermal gelling; PA-PLX-PA; concentration effect
Issue Date
2011-01
Publisher
Soft Matter
Citation
VOL 7, NO 2, 456-462
Abstract
Three dimensional (3D) cell culturing in an artificial matrix needs understanding of the dynamic microenvironments of the extracellular matrix and the cells. In this paper, we investigated a thermal gelling polyalanine–poloxamer–polyalanine (PA–PLX–PA) aqueous solution for chondrocyte 3D culture, focusing on the initial concentration of the polymer aqueous solution. As the polymer concentration increased from 7.0 wt. % to 10.0 wt. % and 15.0 wt. %, moduli of the in situ formed gels at 37 C were increased from 350–380 Pa to 2100–2300 Pa and 5300–5700 Pa, respectively. In addition, the population and thickness of the nanofibers in the gel were increased. Chondrocytes kept their spherical phenotypes in the 3D environment of the in situ formed PA–PLX–PA hydrogel. They showed excellent cell viability, increased production of sGAG and type II collagen in PA–PLX–PA gel prepared from initial polymer concentration of 7.0 wt. % and 10.0 wt. %, emphasizing the significance of the micromechanical environments for the 3D cell culture.
URI
http://pubs.kist.re.kr/handle/201004/39358
ISSN
1744683X
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KIST Publication > Article
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