Top-down proteomic analysis by use of molecular weight-based separation and online LC-FTMS

Title
Top-down proteomic analysis by use of molecular weight-based separation and online LC-FTMS
Authors
이지은John C. Tran신상미이재익김호준이강미권오승Neil L. Kelleher
Issue Date
2011-02
Publisher
29th Manfred Donike Workshop
Abstract
Based on our previous results for proteomic analysis, the use of GELFrEE followed by online RPLC and ultra-high resolution of Orbitrap mass spectrometer is expected to establish a high-throughput method of analyzing the peptide hormones and protein drugs covering wide molecular weight ranges. Currently, we are analyzing protein standard mixtures from 5 to 30 kDa based on top-down approach by use of online RPLC followed by Orbitrap mass spectrometer and optimizing the instrumental parameters in order to differentiate the protein drugs from endogenous forms. We will apply GELFrEE scheme to fractionate the substances present in urine samples and analyze the spiked protein drugs by use of Orbitrap mass spectrometer as the next step, thus ultimately aiming to establish a robust method of peptide and protein drugs based on MS.
URI
http://pubs.kist.re.kr/handle/201004/39472
Appears in Collections:
KIST Publication > Conference Paper
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