Tumor-homing photosensitizer-conjugated glycol chitosan nanoparticles for synchronous photodynamic imaging and therapy based on cellular on/off system

Title
Tumor-homing photosensitizer-conjugated glycol chitosan nanoparticles for synchronous photodynamic imaging and therapy based on cellular on/off system
Authors
이소진구희범이동은민솔기이슬기Xiaoyuan Chen최용석James F. Leary박기남정서영권익찬김광명최귀원
Keywords
Photosensitizer; Nanoparticle; Photodynamic therapy; Drug delivery; Glycol chitosan; Cellular on-off system
Issue Date
2011-06
Publisher
Biomaterials
Citation
VOL 32, NO 16, 4021-4029
Abstract
Herein, we developed the photosensitizer, protoporphyrin IX (PpIX), conjugated glycol chitosan (GC) nanoparticles (PpIX–GC–NPs) as tumor-homing drug carriers with cellular on/off system for photodynamic imaging and therapy, simultaneously. In order to prepare PpIX–GC–NPs, hydrophobic PpIXs were chemically conjugated to GC polymer and the amphiphilic PpIX–GC conjugates formed a stable nanoparticle structure in aqueous condition, wherein conjugated PpIX molecules formed hydrophobic inner-cores and they were covered by the hydrophilic GC polymer shell. Based on the nanoparticle structure, PpIX–GC–NPs showed the self-quenching effect that is ‘off’ state with no fluorescence signal and phototoxicity with light exposure. It is due to the compact crystallized PpIX molecules in the nanoparticles as confirmed by dynamic light scattering and X-ray diffraction methods. However, after cellular uptake, compact nanoparticle structure gradually decreased to generate strong fluorescence signal and singlet oxygen generation when irradiated. Importantly, PpIX–GC–NPs-treated mice presented prolonged blood circulation, enhanced tumor targeting ability, and improved in vivo therapeutic efficiency in tumor-bearing mice, compared to that of free PpIX-treated mice. These results proved that this tumor-homing cellular ‘on/off’ nanoparticle system of PpIX–GC–NPs has a great potential for synchronous photodynamic imaging and therapy in cancer treatment.
URI
http://pubs.kist.re.kr/handle/201004/39551
ISSN
0142-9612
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KIST Publication > Article
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