Real Time, High Resolution Video Imaging of Apoptosis in Single Cells with a Polymeric Nanoprobe

Title
Real Time, High Resolution Video Imaging of Apoptosis in Single Cells with a Polymeric Nanoprobe
Authors
이슬기최기영정현진류주희이애주구희범윤인찬박재형김인산김상윤Xiaoyuan Chen정서영권익찬김광명최귀원
Issue Date
2011-02
Publisher
Bioconjugate chemistry
Citation
VOL 22, NO 2, 125-131
Abstract
We report a new apoptosis nanoprobe (Apo-NP) designed on the basis of a polymer nanoparticle platform. This simple one-step technique is capable of boosting fluorescence signals upon apoptosis in living cells, enabling real-time imaging of apoptosis in single cells and in vivo. The Apo-NP efficiently delivers chemically labeled, dual-quenched caspase-3-sensitive fluorogenic peptides into cells, allowing caspase-3-dependent strong fluorescence amplification to be imaged in apoptotic cells in real-time and at high resolution. The design platform of the Apo-NP is flexible and can be fine-tuned for a wide array of applications such as identification of caspase-related apoptosis in pathologies and for monitoring therapeutic efficacy of apoptotic drugs in cancer treatment.
URI
http://pubs.kist.re.kr/handle/201004/39556
ISSN
1043-1802
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KIST Publication > Article
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