Chondrogenic Differentiation of Human Adipose Tissue-Derived Stem Cells in Functional PLGA Scaffolds
- Chondrogenic Differentiation of Human Adipose Tissue-Derived Stem Cells in Functional PLGA Scaffolds
- 박재구; 이정호; 김정남; 강조아; 김기주; 박귀덕; 한동근; 안상태; 이종원
- chondrogenesis; scaffold; differentiation; adipose derived stem cells (ADSCs)
- Issue Date
- Tissue Engineering and Regenerative Medicine
- VOL 8, NO 1, 47-54
- We evaluated the chondrogenic potential of human adipose tissue-derived stem cells (hADSCs) in a
functional PLGA scaffold containing two chondrogenic factors, dexamethasone and transforming growth factor-α1.
In the functional scaffold group, hADSCs were seeded into functional PLGA scaffolds and cultured with complete
medium. In the positive control group, hADSCs were seeded into a conventional PLGA scaffold and cultured with
chondrogenic medium. In the negative control group, cells in a conventional PLGA scaffold were cultured with
complete medium. After four weeks of differentiation, the expression of cartilage-specific genes and proteins was
evaluated by reverse transcriptase polymerase chain reaction (RT-PCR) and western blotting. Alcian blue and Safranin
O stain were performed to confirm chondrogenic differentiation. Both functional PLGA scaffolds and conventional
PLGA scaffolds seeded with hADSCs were also transplanted into the subcutaneous pockets of athymic mice.
After four weeks, chondrogenesis was analyzed by RT-PCR and western blotting for collagen type II. Alcian blue
stain was also performed. The results of our in vitro study show that a functional PLGA scaffold is as efficient at
stimulating chondrogenic differentiation as the positive control. The results of our in vivo study show that chondrogenic
differentiation is significantly up-regulated with functional PLGA scaffolds. In conclusion, we show that a
functional PLGA scaffold can trigger the chondrogenic differentiation of hADSCs in vitro without chondrogenic
factors. Furthermore, we show that chondrogenic differentiation of hADSCs can be maintained in vivo through the
in situ release of chondrogenic factors.
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