Solvent composition effects on efficiency of pressurized liquid extraction of bioactive isoflavonoids from Belamcanda chinensis rhizomes
- Solvent composition effects on efficiency of pressurized liquid extraction of bioactive isoflavonoids from Belamcanda chinensis rhizomes
- 이희주; 이은하; 정상훈; 이동언; 김철영
- Belamcanda chinensis; isoflavonoids; pressurized liquid extraction,; tectoridin; tectorigenin; thermal stability
- Issue Date
- Journal of liquid chromatography & related technologies
- VOL 34, NO 2, 143-154
- A pressurized liquid extraction (PLE) technique was developed for the extraction of bioactive
isoflavonoids including tectoridin (1), iridin (2), tectorigenin (3), iristectorigenin A (4), irisflorentine
(5), and irigenin (6) fromBelamcanda chinensis using aqueous ethanol as the extraction solvent.
The effects of various key factors of PLE, including solvent composition (0–100% ethanol in water),
temperature (80–180℃) and extraction time (5–20 min), were evaluated with respect to the extraction
efficiency. Only solvent compositions altered the extraction yields of isoflavonoids with 60% ethanol
providing the best extraction efficiency. When compared with conventional extraction methods,
such as reflux or sonication extraction, PLE resulted in higher extraction efficiency with shorter extraction
time and lower solvent consumption.We also tested the thermal stability of tectoridin, isoflavonoid
glycoside, at various temperatures (80–180℃) in 60% ethanol for a 20min extraction time to mimic
conditions that could be encountered during PLE. Tectoridin was degraded to tectorigenin (aglycone)
at temperatures over 150℃, therefore, care should be taken in the choice of temperature used for PLE.
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