Hydrophilic interaction chromatography-tandem mass spectrometry method for identification and quantitation of 5-MeO-DIPT and its metabolites in rat urine

Title
Hydrophilic interaction chromatography-tandem mass spectrometry method for identification and quantitation of 5-MeO-DIPT and its metabolites in rat urine
Authors
김윤김운용인문교이재익권오승유혜현
Keywords
5-MeO-DIPT; HILIC-MS/MS; Metabolites; Profiling; Quantitation
Issue Date
2011-04
Publisher
Bulletin of the Korean Chemical Society
Citation
VOL 32, NO 4, 1158-1164
Abstract
5-Methoxy-N,N-diisopropyltryptamine (5-MeO-DIPT), a psychoactive tryptamine derivative, is a hallucinogenic drug of abuse. In this study, 5-OH-DIPT and its metabolites were identified and the quantitative method was developed and validated by using hydrophilic interaction chromatography-tandem mass spectrometry (HILICMS/ MS). Chromatographic separation was achieved on an Atlantis HILIC silica column (5 μm, 100 ×2.1 mm). The metabolites of 5-MeO-DIPT in rat urine were characterized via Q1 scanning and product ion scanning. As a consequence, 5-MeO-IPT, 5-OH-DIPT, 6-OH-5-MeO-DIPT and their glucuronide conjugates were detected and identified as the metabolites of 5-MeO-DIPT. Subsequently, a quantitative method for 5-MeO-DIPT and its major metabolites, 5-MeO-IPT and 5-OH-DIPT, was developed in multiple reactions monitoring (MRM) mode. The calibration curves for all analytes evidenced good linearity over the concentration range of 1- 1000 ng/mL with linear correlation co-efficients (r2) in excess of 0.99. The intra- and inter-day accuracy and precision were 92.2-110.2% and 1.5-9.9%, respectively.
URI
http://pubs.kist.re.kr/handle/201004/39658
ISSN
0253-2964
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KIST Publication > Article
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