Global metabolic profiling on EAE using UPLC-QTOF-MS

Global metabolic profiling on EAE using UPLC-QTOF-MS
Issue Date
The second Korea Metabolomics Society Symposium
Autoimmune diseases arise from an overactive immune response of the body against substances and tissues normally present in the body. EAE as a model for autoimmune inflammatory diseases of the central nervous system (CNS). EAE in the mouse shares some clinical features with the human disease multiple sclerosis (MS), most prominently paralysis and ataxia. In this study metabolic alternation in EAE was investigated through global metabolic profiling to characterize EAE. C57BL/6 female mice were injected with MOG (Myelin Oligodendrocyte Glycoprotein peptide; 1.5mg/ml) emulsified in Complete Freund’s Adjuvant (CFA) and pertussis toxin (PTX) in PBS. Urine from three mice was collected every 10 day for 57 days. The global analysis of urine sample was performed using ultra performance liquid chromatography/time of flight/mass spectrometry (UPLC-QTOF-MS) with multivariate data analysis. The metabolic differences between control and the EAE mice were comfrimed through OPLS-DA score plots and Hierarchical clustering. Urinary profiling of metabolites related to inflammation and neurotransmittion might be significantly changed in EAE mice. The specific change of metabolic profile might be useful indicator to characterize the EAE models. In addition, this study can indicate that metabolomic approach is valuable to reveal the mechanism of disease.
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