A methylene chloride fraction of Saururus chinensis induces apoptosis through the activation of caspase-3 in prostate and breast cancer cells
- A methylene chloride fraction of Saururus chinensis induces apoptosis through the activation of caspase-3 in prostate and breast cancer cells
- Kim Han-Young; Choi Tae Won; Kim Hyun Jung; Kim Sung-Moo; Park Kyung-Ran; Jang Hyeung-Jin; 이은하; 김철영; 정상훈; Shim Bum Sang; Ahn Kwang Seok
- Saururus chinensis; apoptosis; cancer; caspase-3
- Issue Date
- Phytomedicine : international journal of phytotherapy and phytopharmacology
- VOL 18, NO 7, 567-574
- The aerial parts of Saururus chinensis (SC) have been used for the treatment of edema, fever, jaundice,
and inflammatory diseases in Korean folk medicine for centuries. However, the mechanism by which
SC exerts these anti-tumorigenic activities in human prostate and breast cancer cells has not yet been
fully understood. In this study, we report on the methylene chloride fraction from SC exerting cytotoxicity
against prostate and breast cancer cells in a dose-dependent manner. Specifically, SC exerted the
most potent cytotoxicity in LNCaP and MCF-7 cells. SC was shown to down-regulate various angiogenetic
(VEGF), proliferative (Cyclin D1), anti-apoptotic (Bcl-2) gene products in these cells. SC also increased the
number of annexin V-positive apoptotic bodies and the sub-G1 DNA contents of the cell cycle undergoing
apoptosis through caspase-3 activation in both LNCaP and MCF-7 cells. We further confirmed that
caspase-3 plays an important role in SC-induced apoptosis in LNCaP and MCF-7 cells through the use
of the caspase-3 inhibitor. Moreover, we observed that SC potentiated paclitaxel-induced apoptosis in
MCF-7 cells and sauchinone is a major active constituent of SC, which could induce apoptosis in the cells.
Taken together, our data provide the evidence that SC induces apoptosis depending on caspase-3 activation
and overcomes the natural biological resistance to chemotherapy found in human prostate and
breast cancer cells.
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