Effects of substrate concentration, pH, and temperature on fermentative butanol production from glycerol by Clostridium pasteurianum DSM 525
- Effects of substrate concentration, pH, and temperature on fermentative butanol production from glycerol by Clostridium pasteurianum DSM 525
- 엄영순; 안재형; 상병인
- butanol; fermentation; glycerol
- Issue Date
- 2011 한국생물공학회 춘계학술대회
- Glycerol is a by-product of biodiesel production and ethanol fermentation. In this study, the production of butanol from glycerol by Clostridium pasteurianum DSM525 was evaluated at various substrate concentrations, pHs, and temperatures. The all three factors influenced the growth rate and fermentation product profile. At the glycerol concentration more than 80 g/L, the growth rate and butanol yield were decreased significantly. At the pH values lower than 6.0 or higher than 7.0, the growth rate and butanol yield were decreased significantly and the butanol yield was the highest at pH 6.0 (0.68 mol glycerol converted/mol glycerol consumed). The growth rate was decreased as the temperature decreased from 37 to 25 ℃, and butanol yield was lower at 25 ℃ (0.43) compared with higher temperatures. The butanol yields at 30 and 37 ℃ were similar (0.68 and 0.63, respectively), but glycerol consumption was lower at 37 ℃ than at 30 ℃ (56 and 68 g/L, respectively), and thus the final butanol concentration was higher at 30 ℃ than at 37 ℃. At the optimal condition (pH 6.0, 30 ℃ and 80 g/L of glycerol), C. pasteurianum DSM 525 produced 18.3 g/L of butanol for 36-hour incubation.
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