Isolation of mutants in Clostridium tyrobutyricum for improved tolerance to lignin
- Isolation of mutants in Clostridium tyrobutyricum for improved tolerance to lignin
- 이경민; 김기연; 민경선; 조민호; 한성옥; 엄영순; 상병인
- C. tyrobutyricum; Gamma-ray irradiation; lignin
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- Recently, biomass is regarded as one of the important resources because that can be converted to biofuels which is the most interested issue in the field of biotechnology. Many biochemical processes have adapted saccharificated land plants and microorganisms such as Clostridium tyrobutyricum to produce biofuels. In the process, most of the microorganism convert the lignocellulose hydrolysate to bioenergy (butyric acid) under strict anaerobic condition. The land plants contains about 26% lignin (phenolics) in general. However, lignin (phenolics) often inhibits the cell growth and consequently the productivity in the process. In this study, we isolated mutated Clostridium tyrobutyricum that have tolerance to lignin by gamma-ray irradiation. These mutants exhibited the remarkably improved cell growth and the production of butyric acid to the level of the control.
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