Rapid identification and evaluation of antioxidant compounds from extracts of Petasites japonicus by hyphenated-HPLC techniques

Title
Rapid identification and evaluation of antioxidant compounds from extracts of Petasites japonicus by hyphenated-HPLC techniques
Authors
김상민강석우전제승정유진김철영판철호엄병헌
Keywords
hyphenated techniques; LC/UV-MS; on-line antioxidant activity; Petasites japonicus; radical scavenging acitivity; LC-NMR
Issue Date
2012-02
Publisher
Biomedical chromatography : BMC
Citation
VOL 26, NO 2, 199-207
Abstract
Gradient HPLC coupled to DAD, MS/MS and NMR was applied to the rapid structure determination of major compounds of methanol extracts from leaves and roots of Petasites japonicus. The relative antioxidant capacities of the compounds were evaluated by an HPLC system with post‐column on‐line antioxidant detection based on 2,2′‐azinobis‐3‐ ethylbenzothiazoline‐6‐sulfonic acid radical scavenging. Six compounds were successfully separated on a reverse‐phase C18 column and were identified as 5‐caffeoylquinic acid (5‐CQA), fukinolic acid (FA), 3,5‐di‐O‐caffeoylquinic acid (3,5‐DCQA), quercetin‐3‐O‐(6″‐acetyl)‐β‐glucopyranoside (QAG), 4,5‐di‐O‐caffeoylquinic acid (4,5‐DCQA) and kaempferol‐3‐O‐(6″‐acetyl)‐β‐ glucopyranoside (KAG) by MS/MS and 1H NMR data. Among these compounds, those containing a caffeoyl moiety (5‐CQA, FA, 3, 5‐ and 4,5‐DCQA) showed relatively strong radical scavenging capacity, with 3,5‐DCQA having the greatest radical scavenging capacity in leaf (23.09%of total antioxidant capacity) and root (26.47%) extracts. The relative radical scavenging portion of QAG was only 3.41% in the leaves and KAG did not show any radical scavenging activity. These results demonstrate that the hyphenatedHPLC techniques can be successfully applied to rapidly identify structures and evaluate antioxidant activitieswithout prior purification of compounds from plant tissues of P. japonicus.
URI
http://pubs.kist.re.kr/handle/201004/39758
ISSN
0269-3879
Appears in Collections:
KIST Publication > Article
Files in This Item:
There are no files associated with this item.
Export
RIS (EndNote)
XLS (Excel)
XML


qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

BROWSE