Edible Wild Vegetable, Gymnaster koraiensis Protects Retinal Ganglion Cells Death Caused by Oxidative Stress

Title
Edible Wild Vegetable, Gymnaster koraiensis Protects Retinal Ganglion Cells Death Caused by Oxidative Stress
Authors
정상훈김경아강규동이은하노주원
Issue Date
2011-05
Publisher
The Association for Research in Vision and Ophthalmology
Abstract
Purpose: This study was to determine edible wild vegetable, Gymnaster koraiensis, is effective at blunting the negative influence of N-methyl-D-aspartate (NMDA) to the rat and of oxidative stress induced transformed retinal ganglion cells (RGC-5 cells) death. Methods: RGC-5 cells in culture were given negative insults such as 300 µM H2O2 for 24 h, after which cell survival were tested. ROS quantification were performed by using the fluorescence probe, 2΄,7΄-dichlorodihydrofluorescein diacetate (DCFH–DA) , and we tested glutathione level based on the reaction of glutathione with 5,5′-dithio-bis(2-nitrobenzoic acid) (DTNB). Apoptotic cell death were measured by propidium iodide (PI)/Hoechst 33342 double staining and western blot analysis. NMDA-induced retinal damage in vivo was tested by hematoxylin-eosin staining (H&E) and terminal deoxynucleotidyl transferase-mediated dUTP nick-end labelling (TUNEL) staining. The amount of formation for thiobarbituric acid reactive species (TBARS) was used as the rate of membrane lipid peroxidation. Results: The ethyl acetate fraction of G. koraiensis (EAGK) significantly attenuated H2O2 to RGC-5 cells. Treatment of the RGC-5 cells with EAGK reduced the reactive oxygen species (ROS) and recovered reduced glutathione levels caused by various radical species such as H2O2, OH∙ or O2.-. Moreover, EAGK inhibited lipid peroxidation. Conclusions: EAGK has neuroprotective effects in vitro and in vivo.
URI
http://pubs.kist.re.kr/handle/201004/39815
Appears in Collections:
KIST Publication > Conference Paper
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