Edible Wild Vegetable, Gymnaster koraiensis Protects Retinal Ganglion Cells Death Caused by Oxidative Stress
- Edible Wild Vegetable, Gymnaster koraiensis Protects Retinal Ganglion Cells Death Caused by Oxidative Stress
- 정상훈; 김경아; 강규동; 이은하; 노주원
- Issue Date
- The Association for Research in Vision and Ophthalmology
- Purpose: This study was to determine edible wild vegetable, Gymnaster koraiensis, is effective at blunting the negative influence of N-methyl-D-aspartate (NMDA) to the rat and of oxidative stress induced transformed retinal ganglion cells (RGC-5 cells) death.
Methods: RGC-5 cells in culture were given negative insults such as 300 µM H2O2 for 24 h, after which cell survival were tested. ROS quantification were performed by using the fluorescence probe, 2΄,7΄-dichlorodihydrofluorescein diacetate (DCFH–DA) , and we tested glutathione level based on the reaction of glutathione with 5,5′-dithio-bis(2-nitrobenzoic acid) (DTNB). Apoptotic cell death were measured by propidium iodide (PI)/Hoechst 33342 double staining and western blot analysis.
NMDA-induced retinal damage in vivo was tested by hematoxylin-eosin staining (H&E) and terminal deoxynucleotidyl transferase-mediated dUTP nick-end labelling (TUNEL) staining. The amount of formation for thiobarbituric acid reactive species (TBARS) was used as the rate of membrane lipid peroxidation.
Results: The ethyl acetate fraction of G. koraiensis (EAGK) significantly attenuated H2O2 to RGC-5 cells. Treatment of the RGC-5 cells with EAGK reduced the reactive oxygen species (ROS) and recovered reduced glutathione levels caused by various radical species such as H2O2, OH∙ or O2.-. Moreover, EAGK inhibited lipid peroxidation.
Conclusions: EAGK has neuroprotective effects in vitro and in vivo.
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