Antifungal activity of ethanol extract and its fractions from Hosta capitata on human pathogenic and airborne fungi

Title
Antifungal activity of ethanol extract and its fractions from Hosta capitata on human pathogenic and airborne fungi
Authors
윤지호이샛별이희주김철영노주원
Keywords
Hosta capitata; antifungal activity; Candida albicans; Saccharomyces serevisiae; Cladosporium cladosporioides
Issue Date
2011-05
Publisher
2011 한국생화학분자생물학회 연례국제학술대회
Abstract
Human pathogenic fungi usually induce pathogenic diseases through infection to body. Especially, the airborn fungi, their spores are claimed to cause many diseases such as allergic, respiratory diseases, and inflammation. In this study, we investigated the antifungal activity of extract and its fractions from Hosta capitata against human pathogenic fungi including C. albicans and S.cerevisiae as well as airborne fungi such as C. cladosporeoides. We found that the extract and BuOH fraction showed potent antifungal activity against three fungi tested by both agar diffusion method and minimum inhibitory concentration assay. Moreover, this extract and BuOH fraction showed the inhibition of dimorphic transition in C. albicans which is a key mechanism in the pathogenesis of host infection, was confirmed by fetal bovine serum inducing assay. To elucidate the mode of action for growth inhibition against C. albicans and S.cerevisiae, we performed a flow cytometric cell cycle analysis and found cell cycle arrest by either the extract or BuOH treatment. Taken together, Hosta capitata extract and its BuOH fraction exert the antifungal activation on human pathogenic and airborne fungi, suggesting a good candidate for potent natural antifungal agent.
URI
http://pubs.kist.re.kr/handle/201004/39832
Appears in Collections:
KIST Publication > Conference Paper
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