Statistical optimization of medium compositions toward butanol production from glycerol using Clostridium pasteurianum DSM525

Title
Statistical optimization of medium compositions toward butanol production from glycerol using Clostridium pasteurianum DSM525
Authors
문철우상병인김승욱엄영순
Issue Date
2011-05
Publisher
34th Symposium on Biotechnology for Fuels and Chemicals
Abstract
The medium compositions favoring butanol and 1,3-propanediol (1,3-PDO) production from glycerol fermentation by Clostridium pasteurianum DSM525 were investigated using statistical experimental designs. Medium components significantly affecting butanol and 1,3-PDO production were screened using fractional factorial experimental design. Among the tested six variables (phosphate buffer, MnSO4∙H2O, MgSO4∙7H2O, FeSO4∙7H2O, (NH4)2SO4, yeast extract), FeSO4∙7H2O, (NH4)2SO4, and yeast extract were selected to be significant variables for further optimization of medium using a Box-Behnken design. The optimal butanol productivity (0.98 gL-1h-1) and the 1,3-PDO productivity (1.19 gL-1h-1) were predicted by the corresponding quadratic model for each product. The models were validated by experimentally obtained butanol productivity (1.01 gL-1h-1) and 1,3-PDO productivity (1.14 gL-1h-1) under the optimized conditions. The optimal medium composition for butanol production was significantly different from that for 1,3-PDO production, suggesting that the product formation from glycerol fermentation by C. pasteurianum DSM525 can be controlled by changing medium compositions.
URI
http://pubs.kist.re.kr/handle/201004/39846
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KIST Publication > Conference Paper
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