Statistical optimization of medium compositions toward butanol production from glycerol using Clostridium pasteurianum DSM525
- Statistical optimization of medium compositions toward butanol production from glycerol using Clostridium pasteurianum DSM525
- 문철우; 상병인; 김승욱; 엄영순
- Issue Date
- 34th Symposium on Biotechnology for Fuels and Chemicals
- The medium compositions favoring butanol and 1,3-propanediol (1,3-PDO) production from glycerol fermentation by Clostridium pasteurianum DSM525 were
investigated using statistical experimental designs. Medium components significantly affecting butanol and 1,3-PDO production were screened using fractional factorial
experimental design. Among the tested six variables (phosphate buffer, MnSO4∙H2O, MgSO4∙7H2O, FeSO4∙7H2O, (NH4)2SO4, yeast extract), FeSO4∙7H2O, (NH4)2SO4, and
yeast extract were selected to be significant variables for further optimization of medium using a Box-Behnken design. The optimal butanol productivity (0.98 gL-1h-1)
and the 1,3-PDO productivity (1.19 gL-1h-1) were predicted by the corresponding quadratic model for each product. The models were validated by experimentally
obtained butanol productivity (1.01 gL-1h-1) and 1,3-PDO productivity (1.14 gL-1h-1) under the optimized conditions. The optimal medium composition for butanol
production was significantly different from that for 1,3-PDO production, suggesting that the product formation from glycerol fermentation by C. pasteurianum DSM525
can be controlled by changing medium compositions.
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