Effects of culture conditions on the fermentative production of butanol from glycerol by Clostridium pasteurianum DSM 525
- Effects of culture conditions on the fermentative production of butanol from glycerol by Clostridium pasteurianum DSM 525
- 안재형; 상병인; 엄영순
- glycerol; butanol; clostridium
- Issue Date
- 34th Symposium on Biotechnology for Fuels and Chemicals
- Batch fermentation was performed to investigate the effects of pH and temperature on the butanol production of Clostridium pasteurianum DSM 525 using a synthetic medium
containing glycerol as a sole source of carbon and energy. C. pasteurianum DSM 525 showed a good growth at the initial glycerol concentration of 40 g/L, the temperature of 37 ℃,
and a pH range from 5.0 to 6.5. The growth rate and the proportion of butanol among the fermentation products increased as the culture pH decreased, but the final glycerol
consumption decreased at lower pHs, probably due to the reduced tolerance to butanol at lower pHs. The growth rate and the proportion of butanol increased as the temperature of
the medium increased from 25, to 30, and to 37 ℃. However, more butanol was produced at lower temperatures (18.7, 16.8, and 11.5 g/L of butanol were finally produced at the
temperatures of 25, 30, and 37 ℃, respectively), probably due to the reduced toxicity of butanol at lower temperatures. These results indicate that there is no single pH and
temperature condition to maximize the titre, yield, and productivity of the butanol production simultaneously.
- Appears in Collections:
- KIST Publication > Conference Paper
- Files in This Item:
There are no files associated with this item.
- RIS (EndNote)
- XLS (Excel)
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.