Effects of culture conditions on the fermentative production of butanol from glycerol by Clostridium pasteurianum DSM 525

Title
Effects of culture conditions on the fermentative production of butanol from glycerol by Clostridium pasteurianum DSM 525
Authors
안재형상병인엄영순
Keywords
glycerol; butanol; clostridium
Issue Date
2011-05
Publisher
34th Symposium on Biotechnology for Fuels and Chemicals
Abstract
Batch fermentation was performed to investigate the effects of pH and temperature on the butanol production of Clostridium pasteurianum DSM 525 using a synthetic medium containing glycerol as a sole source of carbon and energy. C. pasteurianum DSM 525 showed a good growth at the initial glycerol concentration of 40 g/L, the temperature of 37 ℃, and a pH range from 5.0 to 6.5. The growth rate and the proportion of butanol among the fermentation products increased as the culture pH decreased, but the final glycerol consumption decreased at lower pHs, probably due to the reduced tolerance to butanol at lower pHs. The growth rate and the proportion of butanol increased as the temperature of the medium increased from 25, to 30, and to 37 ℃. However, more butanol was produced at lower temperatures (18.7, 16.8, and 11.5 g/L of butanol were finally produced at the temperatures of 25, 30, and 37 ℃, respectively), probably due to the reduced toxicity of butanol at lower temperatures. These results indicate that there is no single pH and temperature condition to maximize the titre, yield, and productivity of the butanol production simultaneously.
URI
http://pubs.kist.re.kr/handle/201004/39850
Appears in Collections:
KIST Publication > Conference Paper
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