Quantitative profiling of eicosanoids in plasma by liquid chromatography-quadrupole/time-of-flight-mass spectrometry
- Quantitative profiling of eicosanoids in plasma by liquid chromatography-quadrupole/time-of-flight-mass spectrometry
- 이수현; 하영완; 이원용; 정봉철; 최만호
- eicosanoids; LC-MS; inflammation
- Issue Date
- HPLC 2011
- Eicosanoids as the metabolites of arachidonic acid regulates vascular function, inflammation, hypertension and other pathologies. A validated quantitative analysis of 30 eicosanoids including 18 prostaglandins (PGs), 4 leukotrienes (LTs), 2 thromboxanes (TXs) and 6 hydroxyeicosatetraenoic acids (HETEs), were developed with liquid chromatography-quadrupole/time-of-flight-mass spectrometry (LC-QTOF-MS). The sensitive and selective method was achieved in negative ionization of the high-resolution selected ion monitoring (HR/SIM) mode. All eicosanoids were separated through an 1.9 μm particle C18 column (50 x 2.1 mm) at 30 ℃ and gradient elution with mobile phase consisted of 0.1 % formic acid in 95 % water (solvent A) and 0.1 % formic acid in 95 % acetonitrile (solvent B) at a flow rate of 0.25 mL/min within 55 min run. The LC-QTOF-MS based eicosanoids analysis was found to be linear ranged from 0.9929 to 0.9994, while the instrument detection limits (IDL) were below 1 ng/mL in all eicosanoids analyzed. The present method could be applicable in the integration with the evaluation of inflammation and cardiovascular diseases.
- Appears in Collections:
- KIST Publication > Conference Paper
- Files in This Item:
There are no files associated with this item.
- RIS (EndNote)
- XLS (Excel)
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.