A quantitative method for simultaneous determination of 5-methoxy-N,N-diisopropyltryptamine and its metabolites in urine using liquid chromatography-electrospray ionization-tandem mass spectrometry
- A quantitative method for simultaneous determination of 5-methoxy-N,N-diisopropyltryptamine and its metabolites in urine using liquid chromatography-electrospray ionization-tandem mass spectrometry
- 김명길; 진창배; 김진영; 인문교; 권오승; 유혜현
- forensic science; 5-MeO-DIPT; 5-OH-DIPT; 5-MeO-IPT; urine; LC⁄ MS⁄ MS
- Issue Date
- Journal of forensic sciences
- VOL 56, NO 4, 1044-1048
- 5-Methoxy-N,N-diisopropyltryptamine (5-MeO-DIPT) is a designer hallucinogen derived from tryptamine and is reportedly abused
and involved in criminal activities. For the detection of 5-MeO-DIPT use, a liquid chromatography–tandem mass spectrometric method for 5-MeODIPT
and its metabolites, 5-hydroxy-N,N-diisopropyltryptamine (5-OH-DIPT) and 5-methoxy-N,N-isopropyltryptamine (5-MeO-IPT) was developed
and validated in rat urine. The urine samples were pretreated by protein precipitation with acetonitrile and introduced into a BDS HYPERSIL C18
column (50 x 2.0 mm, 5 lm) for chromatographic separation. Mobile phases consisted of methanol, water, and 1% formic acid, and gradient elution
was used at a flow rate of 0.2 mL⁄ min. For the MS detection, multiple-reaction monitoring analysis was adopted. The linear range was 0.01–
10 lg ⁄ mL, and the lower limit of quantification was 10 ng ⁄mL for all analytes. The intra- and interday accuracies and precisions met the criteria
(<15%). The developed method was successfully applied to the drug-treated rat urine.
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