A quantitative method for simultaneous determination of 5-methoxy-N,N-diisopropyltryptamine and its metabolites in urine using liquid chromatography-electrospray ionization-tandem mass spectrometry

Title
A quantitative method for simultaneous determination of 5-methoxy-N,N-diisopropyltryptamine and its metabolites in urine using liquid chromatography-electrospray ionization-tandem mass spectrometry
Authors
김명길진창배김진영인문교권오승유혜현
Keywords
forensic science; 5-MeO-DIPT; 5-OH-DIPT; 5-MeO-IPT; urine; LC⁄ MS⁄ MS
Issue Date
2011-07
Publisher
Journal of forensic sciences
Citation
VOL 56, NO 4, 1044-1048
Abstract
5-Methoxy-N,N-diisopropyltryptamine (5-MeO-DIPT) is a designer hallucinogen derived from tryptamine and is reportedly abused and involved in criminal activities. For the detection of 5-MeO-DIPT use, a liquid chromatography&#8211;tandem mass spectrometric method for 5-MeODIPT and its metabolites, 5-hydroxy-N,N-diisopropyltryptamine (5-OH-DIPT) and 5-methoxy-N,N-isopropyltryptamine (5-MeO-IPT) was developed and validated in rat urine. The urine samples were pretreated by protein precipitation with acetonitrile and introduced into a BDS HYPERSIL C18 column (50 x 2.0 mm, 5 lm) for chromatographic separation. Mobile phases consisted of methanol, water, and 1% formic acid, and gradient elution was used at a flow rate of 0.2 mL&#8260; min. For the MS detection, multiple-reaction monitoring analysis was adopted. The linear range was 0.01&#8211; 10 lg &#8260; mL, and the lower limit of quantification was 10 ng &#8260;mL for all analytes. The intra- and interday accuracies and precisions met the criteria (<15%). The developed method was successfully applied to the drug-treated rat urine.
URI
http://pubs.kist.re.kr/handle/201004/40019
ISSN
0022-1198
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