Edible wild vegetable, Gymnaster koraiensis protects retinal ganglion cells against oxidative stress
- Edible wild vegetable, Gymnaster koraiensis protects retinal ganglion cells against oxidative stress
- 김경아; 강규동; 이은하; 노주원; 정상훈
- Gymnaster koraiensis; glaucoma; retinal ganglion cells; 3,5-di-O-caffeoylquinic acid; RGC-5; Neuroprotection
- Issue Date
- Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association
- VOL 49, NO 9, 2131-2143
- This study was conducted to determine whether Gymnaster koraiensis is effective at blunting the negative
influence of N-methyl-D-aspartate (NMDA) on the retinas of rats and on oxidative stress induced cell
death in transformed retinal ganglion cells (RGC-5).
The ethyl acetate fraction of G. koraiensis (EAGK) and the isolated compound, 3,5-di-O-caffeoylquinic
acid (3,5-DCQA), were shown to significantly attenuate the negative effect of H2O2 on the RGC-5 cells
tested by various procedures.
The inclusion of EAGK or 3,5-DCQA in the culture reduced the reactive oxygen species (ROS) and
replenished the reduced glutathione levels caused by various radical species such as H2O2, O2
ㆍ- or ㆍOH.
Moreover, EAGK or 3,5-DCQA inhibited lipid peroxidation caused by sodium nitroprusside (SNP) in rat
From in vivo experiments, the presence of NMDA in the retina affected the thickness of the inner plexiform
layer (IPL) and the terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling (TUNEL)
in positive ganglion cells. EAGK or 3,5-DCQA protected the thinning of the IPL and increased TUNEL
positive cells in the ganglion cell layer (GCL).
Our results clearly demonstrate the neuroprotective effect of EAGK both in vitro and in vivo. Moreover,
3,5-DCQA is suggested to be the active compound of EAGK.
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