Protective Effects of Human Placenta Extract on Cartilage Degradation in Experimental Osteoarthritis
- Protective Effects of Human Placenta Extract on Cartilage Degradation in Experimental Osteoarthritis
- 김준기; 김태훈; 박상원; 김효연; 김상훈; 이성열; 이선미
- Laennec; Human placenta extract; osteoarthritis; monoiodoacetate; glycosaminoglycan; matrix metalloproteinase
- Issue Date
- Biological & pharmaceutical bulletin
- VOL 33, NO 6, 1004-1010
- This study investigated the effect of human placenta extract (HPE) on cartilage degradation in vitro MG-63
cells, articular cartilage explants, and in vivo monoiodoacetate (MIA)-induced osteoarthritis (OA). Matrix metalloproteinase
(MMP)-2 activity was measured in HPE-treated osteoblastic MG-63 cells. Articular cartilage
explants in rabbit were cultured, and the degree of proteoglycan (PG) degradation was assessed by measuring
the amount of glycosaminoglycan (GAG) released into the culture medium. Experimental osteoarthritis was induced
by intra-articular injection of 3 mg MIA in rats. Beginning 14 d post-MIA injection, HPE was administered
intra-articularly once a day for 14 d. The knee joints were assessed by roentgenography, histology, and
gelatinase activity. HPE inhibited PG degradation in articular cartilage explants. HPE significantly reduced
deformity of knee joints and suppressed the histological change in MIA-induced OA. HPE inhibited MMP-2
activity in MG-63 cells. MMP-2 and -9 activities were also reduced in the cartilages of HPE-treated knee joints.
Our results indicate that HPE has therapeutic effects on OA by protecting cartilage.
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