Protective Effects of Human Placenta Extract on Cartilage Degradation in Experimental Osteoarthritis

Title
Protective Effects of Human Placenta Extract on Cartilage Degradation in Experimental Osteoarthritis
Authors
김준기김태훈박상원김효연김상훈이성열이선미
Keywords
Laennec; Human placenta extract; osteoarthritis; monoiodoacetate; glycosaminoglycan; matrix metalloproteinase
Issue Date
2010-03
Publisher
Biological & pharmaceutical bulletin
Citation
VOL 33, NO 6, 1004-1010
Abstract
This study investigated the effect of human placenta extract (HPE) on cartilage degradation in vitro MG-63 cells, articular cartilage explants, and in vivo monoiodoacetate (MIA)-induced osteoarthritis (OA). Matrix metalloproteinase (MMP)-2 activity was measured in HPE-treated osteoblastic MG-63 cells. Articular cartilage explants in rabbit were cultured, and the degree of proteoglycan (PG) degradation was assessed by measuring the amount of glycosaminoglycan (GAG) released into the culture medium. Experimental osteoarthritis was induced by intra-articular injection of 3 mg MIA in rats. Beginning 14 d post-MIA injection, HPE was administered intra-articularly once a day for 14 d. The knee joints were assessed by roentgenography, histology, and gelatinase activity. HPE inhibited PG degradation in articular cartilage explants. HPE significantly reduced deformity of knee joints and suppressed the histological change in MIA-induced OA. HPE inhibited MMP-2 activity in MG-63 cells. MMP-2 and -9 activities were also reduced in the cartilages of HPE-treated knee joints. Our results indicate that HPE has therapeutic effects on OA by protecting cartilage.
URI
http://pubs.kist.re.kr/handle/201004/40146
ISSN
0918-6158
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KIST Publication > Article
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