Protective effect of GCSB-5, an herbal preparation, against peripheral nerve injury in rats
- Protective effect of GCSB-5, an herbal preparation, against peripheral nerve injury in rats
- 김태훈; 윤성진; 이우철; 김준기; 신준식; 이상호; 이선미
- Achyranthis bidentata Blume; Eucommia ulmoides Oliver; GCSB-5; Motor functional recovery; Oxidative stress; Peripheral nerve injury
- Issue Date
- Journal of ethnopharmacology
- VOL 136, NO 2, 297-304
- Aim of the study: GCSB-5 (traditional name: Chungpa-Juhn), an herbal medicine composed of 6 crude
herbs (Saposhnikovia divaricata Schiskin, Achyranthis bidentata Blume, Acanthopanax sessiliflorum Seem,
Cibotium baromets J. Smith, Glycine max Meriill, and Eucommia ulmoides Oliver), has been widely used
in Asia for treatment of neuropathic and inflammatory diseases. This study investigated the protective
effect of GCSB-5 against peripheral nerve injury in vitro and in vivo.
Materials and methods: After left sciatic nerve transection, rats received oral administration of GCSB-5
(30, 100, 300, and 600 mg/kg), or saline (vehicle), respectively, once daily for 8 weeks. Motor functional
recovery and axonal nerve regeneration were evaluated by measurement of sciatic functional index
(SFI), sensory regeneration distance, and gastrocnemius muscle mass ratio. The myelinated axon number
was counted by morphometric analysis. In the in vitro study, the effects of GCSB-5 on H2O2-induced
oxidative damage in SH-SY5Y cells were investigated by measurement of cell viability, production of
reactive oxygen species (ROS), lipid peroxidation, release of lactate dehydrogenease (LDH), and cellular
glutathione contents. Neurite outgrowth was also determined.
Results: After 8 weeks of nerve transection, SFI, regeneration distance, and gastrocnemius muscle mass
ratio and myelinated axon number showed a significant decrease and these decreases were attenuated
by GCSB-5. GCSB-5 significantly inhibited H2O2-induced cell death and oxidative stress, as evidenced by
decreases in production of ROS and lipid peroxidation and release of LDH, and by increase in total GSH
Conclusions: The neuroprotective effect afforded by GCSB-5 is due in part to reduced oxidative stress.
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