NONOates-Polyethylenimine Hydrogel for Controlled Nitric Oxide Release and Cell Proliferation Modulation
- NONOates-Polyethylenimine Hydrogel for Controlled Nitric Oxide Release and Cell Proliferation Modulation
- 김지훈; 이양기; Kaushik Singha; 김현우; 신재호; 조성봉; 한동근; 김원종
- Issue Date
- Bioconjugate chemistry
- VOL 22, NO 6, 1031-1038
- In recent years, numerous research activities have been devoted
to the controlled release of nitric oxide (NO) due to its potential as a restenosis
inhibitor which inhibits the proliferation of vascular smooth muscle cells, the
apoptosis of vascular endothelial cells, and aggregation of platelets. This work
has demonstrated the development of a novel NO-conjugated gel system
comprising of thermosensitive Pluronic F127, branched polyethylenimine
(BPEI), and diazeniumdiolates (NONOates). Synthesis of conjugated Pluronic-
BPEI-NONOates involved coupling of activated F127 to BPEI followed by
the installation of NONOates at the secondary amine sites of branched PEI
backbone under high pressure. NO-conjugated gel system, F127-BPEI-NONOates,
reduced the initial burst of NO release and prolonged NO release.
Furthermore, F127-BPEI-NONOates polymer coated on cell culture dish
displayed much higher increase of endothelial cell proliferation and reduction
of smooth muscle cell proliferation than that exhibited by non-NO releasing control. Such an NO-releasing device can operate
locally and has a great potential in several biomedical applications due to high biocompatibility
imparted by the conjugated F127.
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