Effect of Microfluidization on in Vitro Micellization and Intestinal Cell Uptake of Lutein from Chlorella vulgaris
- Effect of Microfluidization on in Vitro Micellization and Intestinal Cell Uptake of Lutein from Chlorella vulgaris
- 차광현; 이주영; 송대근; 김상민; 이동언; 전진영; 판철호
- Chlorella vulgaris; lutein; microfluidization; bioaccessibility; in vitro digestion; micellization; Caco-2 cells
- Issue Date
- Journal of agricultural and food chemistry
- VOL 59, NO 16, 8670-8674
- Chlorella is a nutrient-rich microalga that contains protein, lipid, minerals, vitamins, and high levels of lutein. This
study evaluated the bioavailability of lutein from Chlorella vulgaris using a coupled in vitro digestion and human intestinal Caco-2 cell
model. Lutein bioaccessibility was low, and approximately 75% of total C. vulgaris lutein was not micellized during the digestion
process but remained in the insoluble digestate. Microfluidization improved lutein micellization efficiency during C. vulgaris
digestion. C. vulgaris was microfluidized at a pressure exceeding 10000 psi, and the cell surface disruption was visualized by scanning
electron microscopy. The mean C. vulgaris particle size was reduced from 3.56 to 0.35 μm with the microfluidization treatment.
C. vulgaris microfluidization at 20000 psi was three times more efficient for aqueous lutein micelles production as compared with
untreated C. vulgaris, and the final lutein content accumulated by intestinal Caco-2 cells was also higher with microfluidization.
C. vulgaris lutein stability was not affected by microfluidization. These results indicate that microfluidization may be useful for
improving lutein bioaccessibility from C. vulgaris during food processing.
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