Enzyme precipitate coatings of lipase on polymer nanofibers

Title
Enzyme precipitate coatings of lipase on polymer nanofibers
Authors
안효진이혜진전승현황상연김병찬김광희이경미오민규김중배
Keywords
Enzyme precipitate coatings; Electrospun polymer nanofibers; Ethanol dispersion; Lipase; Enzyme stabilization
Issue Date
2011-09
Publisher
Bioprocess and biosystems engineering
Citation
VOL 34, NO 7, 841-847
Abstract
Lipase (LP) was immobilized on electrospun and ethanol-dispersed polystyrene–poly(styrene-co-maleic anhydride) (PS–PSMA) nanofibers (EtOH-NF) in the form of enzyme precipitate coatings (EPCs). LP precipitate coatings (EPCs-LP) were prepared in a three-step process, consisting of covalent attachment, LP precipitation, and crosslinking of precipitated LPs onto the covalently attached LPs via glutaraldehyde treatment. The LP precipitation was performed by adding various concentrations of ammonium sulfate (20–50%, w/v). EPCs-LP improved the LP activity and stability when compared to covalently attached LPs (CA-LP) and the enzyme coatings of LPs (EC-LP) without the LP precipitation. For example, the use of 40% (w/v) ammonium sulfate resulted in EPC40-LP with the highest activity, which was 4.0 and 3.6 times higher than those of CA-LP and EC-LP, respectively. After 165-day incubation under rigorous shaking at 200 rpm, the residual activities of EPC50-LP were 0.5 μM/min mg of EtOH-NF, representing 113 and 75 times higher than those of CA-LP and EC-LP, respectively. When LP was partially purified via a simple ammonium sulfate precipitation and dialysis, both activities and stabilities of EC-LP and EPCLP could be marginally improved. It is anticipated that the improved LP activity and stability in the form of EPCs would allow for their potential applications in various bioconversion processes such as biodiesel production and ibuprofen resolution.
URI
http://pubs.kist.re.kr/handle/201004/40193
ISSN
1615-7591
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KIST Publication > Article
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