LC-ESI/MS/MS method for rapid screening and confirmation of 44 exogenous anabolic steroids in human urine

Title
LC-ESI/MS/MS method for rapid screening and confirmation of 44 exogenous anabolic steroids in human urine
Authors
전병욱유혜현정은숙김호준진창배김동현이재익
Keywords
Anabolic steroids; Screening; Confirmation; Doping analysis; Liquid chromatography?andem mass spectrometry
Issue Date
2011-09
Publisher
Analytical and bioanalytical chemistry
Citation
VOL 401, NO 4, 1353-1363
Abstract
A sensitive and rapid method based on liquid chromatography–triple-quadrupole tandem mass spectrometry (LC-MS/MS) with electrospray ionization (ESI) has been developed and validated for the screening and confirmation of 44 exogenous anabolic steroids (29 parent steroids and 15 metabolites) in human urine. The method involves an enzymatic hydrolysis, liquid–liquid extraction, and detection by LC-MS/MS. A triple-quadrupole mass spectrometer was operated in positive ESI mode with selected reaction monitoring (SRM) mode for the screening and product ion scan mode for the confirmation. The protonated molecular ions were used as precursor ions for the SRM analysis and product ion scan. The intraday and interday precisions of the target analytes at concentrations of the minimum required performance levels for the screening were 2-14% and 2-15%, respectively. The limits of detection for the screening and confirmation method were 0.1-10 ng/mL and 0.2-10 ng/mL, respectively, for 44 steroids. This method was successfully applied to analysis of urine samples from suspected anabolic steroid abusers.
URI
http://pubs.kist.re.kr/handle/201004/40196
ISSN
1618-2642
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KIST Publication > Article
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