Induction of New Secondary Metabolites by Microbial Co-Culture Interactions
- Induction of New Secondary Metabolites by Microbial Co-Culture Interactions
- 박현봉; 양현옥; 이강노; 권학철
- Issue Date
- American Society of Pharmacognosy
- , 287-287
- Microbial natural products have provided many novel chemical scaffolds which have been used in modern drug
development. However, as the rate of known compound re-isolation from terrestrial microorganisms has been in
creasing for more than a decade, the need to develop more efficient methods for the discovery of new natural pr
oducts has never been greater. One method for accessing untapped natural product resources is to look for organi
sms in unusual environments. One such environment is found in abandoned mine drainages, which often extrem
ely contaminated by heavy metals and acid. In the harsh conditions of an abandoned mine, microorganisms are l
ikely subjected to substantial levels of competition, which could lead them to develop unique chemical arsenals
such as antibiotics. In order to investigate the chemical potential of microbes growing in this extreme environme
nt, we designed a series of microbial co-culturing experiments on the basis of previously reported mixed microbi
al culture studies. Co-culture of a charcoal mine drainage-derived Gordonia sp. KMC-004 and Streptomyces sp.
KMC-005 led to the isolation of new polyene glucoside, Gostrepic acid (1). This compound was not detected in
monoculture broths of KMC-004 or KMC-005. The structure of 1 is closely related to α-Lipomycin produced by
Streptomyces sp. KMC-005. One interesting difference is the elimination of tetramic acid moiety. In addition, w
e also investigated the secondary metabolites produced from the co-culture of an Streptomyces sp. KMC-006 an
d a Aspergillus fumigatus KMC-901. Addition of the Aspergillus fumigatus into the culture broth of the strain K
MC-006 resulted in the production of a new oxazoline, Aspazoline (2). The results provide further evidence that
microbial co-culture can produce novel biologically relevant molecules.
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