Induction of New Secondary Metabolites by Microbial Co-Culture Interactions

Induction of New Secondary Metabolites by Microbial Co-Culture Interactions
Issue Date
American Society of Pharmacognosy
, 287-287
Microbial natural products have provided many novel chemical scaffolds which have been used in modern drug development. However, as the rate of known compound re-isolation from terrestrial microorganisms has been in creasing for more than a decade, the need to develop more efficient methods for the discovery of new natural pr oducts has never been greater. One method for accessing untapped natural product resources is to look for organi sms in unusual environments. One such environment is found in abandoned mine drainages, which often extrem ely contaminated by heavy metals and acid. In the harsh conditions of an abandoned mine, microorganisms are l ikely subjected to substantial levels of competition, which could lead them to develop unique chemical arsenals such as antibiotics. In order to investigate the chemical potential of microbes growing in this extreme environme nt, we designed a series of microbial co-culturing experiments on the basis of previously reported mixed microbi al culture studies. Co-culture of a charcoal mine drainage-derived Gordonia sp. KMC-004 and Streptomyces sp. KMC-005 led to the isolation of new polyene glucoside, Gostrepic acid (1). This compound was not detected in monoculture broths of KMC-004 or KMC-005. The structure of 1 is closely related to α-Lipomycin produced by Streptomyces sp. KMC-005. One interesting difference is the elimination of tetramic acid moiety. In addition, w e also investigated the secondary metabolites produced from the co-culture of an Streptomyces sp. KMC-006 an d a Aspergillus fumigatus KMC-901. Addition of the Aspergillus fumigatus into the culture broth of the strain K MC-006 resulted in the production of a new oxazoline, Aspazoline (2). The results provide further evidence that microbial co-culture can produce novel biologically relevant molecules.
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