Cell type-specific channelrhodopsin-2 transgenic mice for optogenetic dissection of neural circuitry function

Title
Cell type-specific channelrhodopsin-2 transgenic mice for optogenetic dissection of neural circuitry function
Authors
Shengli ZhaoJonathan T TingHisham E AtallahLi QiuJie TanBernd Gloss조지어거스틴Karl DeisserothMinmin LuoAnn M GraybielGuoping Feng
Issue Date
2011-08
Publisher
Nature methods
Citation
VOL 8, NO 9, 745-752
Abstract
Optogenetic methods have emerged as powerful tools for dissecting neural circuit connectivity, function and dysfunction. We used a bacterial artificial chromosome (BAC) transgenic strategy to express the H134R variant of channelrhodopsin-2, ChR2(H134R), under the control of cell type–specific promoter elements. We performed an extensive functional characterization of the newly established VGAT-ChR2(H134R)-EYFP, ChAT-ChR2(H134R)-EYFP, Tph2-ChR2(H134R)-EYFP and Pvalb(H134R)-ChR2-EYFP BAC transgenic mouse lines and demonstrate the utility of these lines for precisely controlling action-potential firing of GABAergic, cholinergic, serotonergic and parvalbumin-expressing neuron subsets using blue light. This resource of cell type–specific ChR2(H134R) mouse lines will facilitate the precise mapping of neuronal connectivity and the dissection of the neural basis of behavior.
URI
http://pubs.kist.re.kr/handle/201004/40246
ISSN
1548-7091
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