Preparative isolation and purification of seven isoflavones from Belamcanda chinensis
- Preparative isolation and purification of seven isoflavones from Belamcanda chinensis
- 이연실; 김선하; 김진규; 이상현; 정상훈; 임순성
- Belamcanda chinensis; HSCCC; isoflavone; high-speed counter-current chromatography
- Issue Date
- Phytochemical analysis : PCA
- VOL 22, NO 5, 468-473
- Introduction – Isoflavonoids from Belamcanda chinensis are known to have a number of physiological benefits including antiinflammatory,
anti‐angiogenic and anti‐mutagenic properties. However, there have been no reports on the effective
isolation and purification of isoflavonoids from B. chinensis.
Objective – To develop an efficient method for the preparative isolation and purification of isoflavones from B. chinensis by
high‐speed counter‐current chromatography (HSCCC).
Methodology – A two‐step HSCCC isolation method was developed using solvent system of n‐hexane–ethyl acetate–2‐
propanol–methanol–water (5:6:2:3.5:6, v/v) and of ethyl acetate–methanol–water (10:2:9, v/v). FLASH purification system
(45% methanol, isocratic) was also used for further purification. The purities and chemical structures of the isolated
compounds were determined by high‐performance liquid chromatography–photodiode array detection (HPLC‐PDA),
electrospray ionisation‐mass spectrometry (ESI‐MS), 1H‐ and 13C‐nuclear magnetic resonance spectrometry (NMR) and
nuclear overhauser enhancement (NOE).
Results – HSCCC was successfully used for the preparative separation and purification of seven isoflavones, including
tectoridin (145.4 mg, 97.5%), iridin (77.9 mg, 94.0%), irilin D (42.0 mg, 92.0%), tectorigenin (294.1 mg, 98.6%), iristectorigenin
A (86.8 mg, 93.4%), irigenin (141.8 mg, 95.8%) and irisflorentin (73.4 mg, 94.7%) from the rhizomes of B. chinensis.
Conclusion – Two isoflavone glycosides and five isoflavone derivatives were successfully isolated and purified from the crude
methanol extract of dried rhizomes of the B. chinensis by HSCCC.
- Appears in Collections:
- KIST Publication > Article
- Files in This Item:
There are no files associated with this item.
- RIS (EndNote)
- XLS (Excel)
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.