Gypenoside TN-2 ameliorates scopolamine-induced learning deficit in mice
- Gypenoside TN-2 ameliorates scopolamine-induced learning deficit in mice
- Sung-Woon Hong; Jeong-hwa Yang; Eun-Ha Joh; 김형자; Dong-Hyun Kim
- Gynostemma pentaphyllum (Thunb.) Makino Gypenoside TN-2; Memory; Scopolamine; Brain-derived neurotrophic factor
- Issue Date
- Journal of ethnopharmacology
- VOL 134, NO 3, 1010-1013
- Ethnopharmacological relevance: Gynostemma pentaphyllum (Thunb.) Makino (GP, family Cucurbitaceae),
which contains dammarane saponins as its main constituents, is used in China, Japan, and Korea as a
traditional medicine to treat cancer, obesity, arteriosclerosis, asthma and senility.
Aim of the study: To investigate the memory-enhancing effects of GP, Gypenoside TN-2 (TN-2) was isolated
by activity-guided fractionation and administered to scopolamine-induced memory-deficient mice.
Materials and methods: The memory-enhancing effects of TN-2 were evaluated using passive avoidance,
Y-maze, and Morris water maze tests, and the protein expressions of brain-derived neurotrophic factor
(BDNF), cAMP element binding protein (CREB), and p-CREB were determined by immunoblotting.
Results: TN-2 inhibitedmemoryand learning deficits in scopolamine treated mice in the passive avoidance
test. TN-2 (10, 20, and 40 mg/kg, p.o.) significantly inhibited memory and learning deficits in the passive
avoidance test by 40%, 96% and 78%, respectively, and exhibited significant memory-enhancing effects
on the Y-maze test and the Morris water maze test. TN-2 also markedly increased BNDF expression and
activated the transcription factor CREB in the hippocampi of scopolamine-treated mice.
Conclusions: TN-2 may ameliorate memory and learning deficits by activating the CREB-BDNF pathway.
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