Hyaluronidase-Sensitive SPIONs for MR/Optical Dual Imaging Nanoprobes
- Hyaluronidase-Sensitive SPIONs for MR/Optical Dual Imaging Nanoprobes
- 이동은; 김아영; Gurusamy Saravanakum; 구희범; 권익찬; 최귀원; 박재형; 김광명
- SPION; hyaluronic acid; hyaluronidase; MR/optical dual imaging
- Issue Date
- Macromolecular research
- VOL 19, NO 8, 861-867
- This study developed new enzyme-specific superparamagnetic iron oxide nanoparticles (SPIONs) with
potential as a multimodal probe in magnetic resonance (MR) imaging and optical imaging. For the MR/optical dual
imaging, SPIONs were coated with near-infrared fluorescence dye (Cy5.5)-labeled hyaluronic acid (Cy5.5-HA),
which could be readily degraded by hyaluronidase (HAdase). The presence of HA on the coated SPION was confirmed
using FTIR, X-ray diffraction, and thermogravimetric analysis. Physicochemical analyses of Cy5.5-HAcoated
SPIONs (Cy5.5-HA-SPIONs) demonstrated their higher colloidal stability and T2 relaxivity than bare SPIONs.
When Cy5.5-HA-SPIONs were exposed to the MR instrument for the phantom test, the dark intensity of their
T2-weighted image increased in a Fe concentration-dependent manner, suggesting potential as the MR probe. To
examine their ability as the enzyme-sensitive optical probe, the fluorescent signals of Cy5.5-HA-SPIONs were measured
in the absence or presence of HAdase. In the absence of HAdase, Cy5.5-HA-SPIONs showed a negligible fluorescent
signal due to possible quenching between a SPION and fluorescence dye, and self-quenching effect of
Cy5.5 molecules. On the other hand, in the presence of HAdase, the fluorescent signal of Cy5.5-HA-SPIONs
increased dramatically as a function of the HAdase concentration, resulting from the degradation of the HA backbone.
These results suggest that the Cy5.5-HA-SPION is useful as a multimodal nanoprobe for MR imaging and
HAdase-sensitive optical imaging.
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