Screening analysis of 10 adrenal steroids by matrix-assisted laser desorption ionization-tandem mass spectrometry

Title
Screening analysis of 10 adrenal steroids by matrix-assisted laser desorption ionization-tandem mass spectrometry
Authors
김선주정현진정봉철최만호
Keywords
adrenal steroid; mass spectrometry; MALDI-MS; inborn error; Congenital adrenal hyperplasia; Newborn screening
Issue Date
2011-09
Publisher
Mass Spectrometry Letters
Citation
VOL 2, NO 3, 69-72
Abstract
Defective synthesis of the steroid hormones by the adrenal cortex has profound effects on human development and homeostasis. Due to the time-consuming chromatography procedure combined with mass spectrometry, the matrix-assisted laser desorption ionization method coupled to the linear ion-trap tandem mass spectrometry (MALDI-LTQ-MS/MS) was developed for quantitative analysis of 10 adrenal steroids in human serum. Although MALDI-MS can be introduced for its applicability as a high-throughput screening method, it has a limitation on reproducibility within and between samples, which renders poor reproducibility for quantification. For quantitative MALDI-MS/MS analysis, the stable-isotope labeled internal standards were used and the conditions of crystallization were tested. The precision and accuracy were 3.1~35.5% and 83.8~138.5%, respectively, when a mixture of 10 mg/mL α-cyano-4-hydroxycinnamic acid in 0.2% TFA of 70% acetonitrile was used as the MALDI matrix. The limit of quantification ranged from 5 to 340 ng/mL, and the linearity as a correlation coefficient was higher than 0.988 for all analytes in the calibration range. Clinical applications include quantitative analyses of patients with congenital adrenal hyperplasia. The devised MALDI-MS/MS technique could be successfully applied to diagnosis of clinical samples.
URI
http://pubs.kist.re.kr/handle/201004/40366
ISSN
2093-8950
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KIST Publication > Article
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