Characterization and identification of lipid-producing microalgae species isolated from a freshwater lake

Title
Characterization and identification of lipid-producing microalgae species isolated from a freshwater lake
Authors
Reda A.I. Abou-ShanaIbrahim A. Matter김수남You-Kwan Oh최재영Byong-Hun Jeon
Keywords
Biodiesel; Freshwater; Isolation; LSU rDNA (D1-D2); Microalgae
Issue Date
2011-10
Publisher
Biomass & bioenergy
Citation
VOL 35, NO 7, 3079-3085
Abstract
Microalgal lipids are the oils of the future for sustainable biodiesel production. One of the most important decisions in obtaining oil from microalgae is the choice of species. A total of 45 algal cultures were isolated from a freshwater lake at Wonju, South Korea. Five microalgal isolates were selected based on their morphology and ease of cultivation under our test conditions. These cultures were identified as strains of Scenedesmus obliquus YSL02, Chlamydomonas pitschmannii YSL03, Chlorella vulgaris YSL04, S. obliquus YSL05, and Chlamydomonas mexicana YSL07 based on microscopic examination and LSU rDNA (D1-D2) sequence analysis. S. obliquus YSL02 reached a higher biomass concentration (1.84 ± 0.30 g L-1) with a lower lipid content (29% w/w), than did Chla. pitschmannii YSL03 (maximum biomass concentration of 1.04 ± 0.09 with a 51% lipid content). Our results suggest that Chla. pitschmannii YSL03 is appropriate for producing biodiesel based on its high lipid content and oleic acid proportion.
URI
http://pubs.kist.re.kr/handle/201004/40391
ISSN
0961-9534
Appears in Collections:
KIST Publication > Article
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