Autodisplay of streptavidin

Title
Autodisplay of streptavidin
Authors
박민Joachim JoseSarah Thommes김조일강민정변재철
Keywords
Autodisplay; Streptavidin
Issue Date
2011-04
Publisher
Enzyme and microbial technology
Citation
VOL 48, NO 4-5, 307-311
Abstract
Streptavidin was expressed on the outer membrane of E. coli as a recombinant fusion protein with an autotransporter domain called AIDA-I (adhesin involved in diffuse adherence) using autodisplay technology. The autodisplay of streptavidin was confirmed by SDS-PAGE of the outer membrane proteins, and the number of autodisplayed streptavidin molecules on a single E. coli cell was evaluated with densitometric analysis. The biotin-binding activity of the autodisplayed streptavidin was estimated after treatment with fluorescently labeled biotin by fluorescence microscopy and flow cytometry. The biotin-binding activity of the E. coli with autodisplayed streptavidin was compared with the activity of streptavidin immobilized on magnetic beads. Finally, the outer membrane presenting autodisplayed streptavidin was isolated and layered on a 96-well microplate for an immunoassay.
URI
http://pubs.kist.re.kr/handle/201004/40498
ISSN
0141-0229
Appears in Collections:
KIST Publication > Article
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