Autodisplay of streptavidin
- Autodisplay of streptavidin
- 박민; Joachim Jose; Sarah Thommes; 김조일; 강민정; 변재철
- Autodisplay; Streptavidin
- Issue Date
- Enzyme and microbial technology
- VOL 48, NO 4-5, 307-311
- Streptavidin was expressed on the outer membrane of E. coli as a recombinant fusion protein with an
autotransporter domain called AIDA-I (adhesin involved in diffuse adherence) using autodisplay technology.
The autodisplay of streptavidin was confirmed by SDS-PAGE of the outer membrane proteins, and the
number of autodisplayed streptavidin molecules on a single E. coli cell was evaluated with densitometric
analysis. The biotin-binding activity of the autodisplayed streptavidin was estimated after treatment with
fluorescently labeled biotin by fluorescence microscopy and flow cytometry. The biotin-binding activity
of the E. coli with autodisplayed streptavidin was compared with the activity of streptavidin immobilized
on magnetic beads. Finally, the outer membrane presenting autodisplayed streptavidin was isolated and
layered on a 96-well microplate for an immunoassay.
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