Rapid Detection of Antioxidant Flavonoids in Azalea (Rhododendron mucronulatum) Flowers using On-line HPLC-ABTS+ System and Preparative Isolation of Three Flavonoids by Centrifugal Partition Chromatography

Title
Rapid Detection of Antioxidant Flavonoids in Azalea (Rhododendron mucronulatum) Flowers using On-line HPLC-ABTS+ System and Preparative Isolation of Three Flavonoids by Centrifugal Partition Chromatography
Authors
전제승김학기엄병헌김철영
Keywords
antioxidant; awalea flowers; centrifugal partition chromatography; flavonols; on-line HPLC-ABTS+ system
Issue Date
2011-05
Publisher
Separation science and technology
Citation
VOL 46, NO 3, 518-524
Abstract
High-performance liquid chromatography coupled to an on-line ABTSþ-based assay (on-line HPLC-ABTSþ) system was used to determine the principle antioxidants in azalea flowers. Three flavonoids, myricetin, quercetin, and kaempferol, recovered in ethyl acetate extracts of azalea flowers were determined to have antioxidant activities. These three flavonoids were isolated and purified by successive centrifugal partition chromatography (CPC) using two different biphasic solvent systems, consisting of n-hexane-ethyl acetate-methanol-water (3:5:3:5 or 4:5:4:5, v/v). A total of 46.2 mg of myricetin, 28.9 mg of quercetin, and 10.6 mg of kaempferol with purities of 97.0%, 95.4%, and 93.9%, respectively, were purified from 500 mg of ethyl acetate soluble material from azalea flowers. The structures were identified by their retention time, UV spectra, and ESI-MS in the positive ion mode and were confirmed by NMR experiments.
URI
http://pubs.kist.re.kr/handle/201004/40583
ISSN
0149-6395
Appears in Collections:
KIST Publication > Article
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