Real-time measurement of UV-inactivated Escherichia coli bacterial particles by electrospray-assisted UVAPS spectrometry
- Real-time measurement of UV-inactivated Escherichia coli bacterial particles by electrospray-assisted UVAPS spectrometry
- 정재희; 이정은; 배귀남
- Electrospray; Bioaerosol; UVAPS; Real-time characterization; Real-time detection
- Issue Date
- The Science of the total environment
- VOL 409, NO 17, 3249-3255
- The ultraviolet aerodynamic particle sizer (UVAPS) is a novel commercially available aerosol spectrometer for
real-time continuous monitoring of viable bioaerosols, based on fluorescence from livingmicroorganisms. In a
previous study, we developed an electrospray-assisted UVAPS using biological electrospray techniques, which
have the advantage of generating non-agglomerated single particles by the repulsive electrical forces. With
this electrospraying of suspensions containing microorganisms, the analytical system can supply more
accurate and quantitative information about living microorganisms than with conventional aerosolization.
Using electrospray-assisted UVAPS, we investigated the characteristics of bacterial particles with various
viabilities in real-time. Escherichia coli was used as the test microorganism, and its initial viability was
controlled by the degree of exposure to UV irradiation. In the stable cone-jet domain, the particle size
distributions of test bacterial particles remained almost uniform regardless of the degree of UV inactivation.
However, the fluorescence spectra of the bacterial particles changed with the degree of UV inactivation. The
fluorescence characteristics of UV-inactivated bacterial particles tended to show a similar decline with
viability, determined by the sampling and culture method, although the percentage showing fluorescence was
higher than that showing viability.
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