Tyrosinase inhibition by water and ethanol extracts of a far eastern sea cucumber, Stichopus japonicus

Title
Tyrosinase inhibition by water and ethanol extracts of a far eastern sea cucumber, Stichopus japonicus
Authors
Amir Husni전제승엄병헌한남수정동화
Keywords
tyrosinase inhibitors; Sea cucumber; Stichopus japonicus; mixed type; ethyl-α-D-glucopyranoside
Issue Date
2011-07
Publisher
Journal of the science of food and agriculture
Citation
VOL 91, NO 9, 1541-1547
Abstract
BACKGROUND: Tyrosinase plays a key role in hyperpigmentaion and enzymatic browning. The present study was aimed at investigating the inhibitory effects of water and 70% aqueous ethanol extracts of Stichopus japonicus, a sea cucumber long consumed as a tonic food and traditional medicine, on the diphenolase activity of tyrosinase. RESULTS: In the tyrosinase inhibition study, high-performance liquid chromatography completely separated L-3,4- dihydroxyphenylalanine and dopachrome from other compounds present in the extracts, and provided more reliable results than the commonly used spectrophotometry. The ethanol extract (IC50 = 0.49–0.61mgmL−1) showed higher inhibitory activity than thewater extract (IC50 = 1.80–1.99mgmL−1). Enzyme inhibition by the extractswas reversible and ofmixed type. For both extracts, the dissociation constants for binding to free enzyme were significantly smaller than those for binding to enzyme–substrate complex. Ethyl-α-D-glucopyranoside (IC50 = 0.19 mg mL−1), isolated for the first time from sea cucumber, and adenosine (IC50 = 0.13 mg mL−1), were identified as key tyrosinase inhibitors. CONCLUSION:Theseacucumberextractsweredemonstratedtopossessconsiderableinhibitorypotencyagainstthediphenolase activity of tyrosinase, suggesting that the sea cucumbermay be a good source of safe and effective tyrosinase inhibitors.
URI
http://pubs.kist.re.kr/handle/201004/40656
ISSN
0022-5142
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KIST Publication > Article
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