The Optimal Conditions for the Estimation of DNA Methylation levels using High Throughput Microarray derived DNA Immunoprecipitation (MeDIP)-Enrichment in Human Bloods

Title
The Optimal Conditions for the Estimation of DNA Methylation levels using High Throughput Microarray derived DNA Immunoprecipitation (MeDIP)-Enrichment in Human Bloods
Authors
송미경송미최한샘류우인윤지성신찬영Youn-Jung Kim류재천
Keywords
Epigenetics; DNA methylation; Methylated DNA immunoprecipitation (MeDIP); Microarray
Issue Date
2011-08
Publisher
Toxicology and environmental health sciences
Citation
VOL 3, NO 3, 185-192
Abstract
Genomic-wide mapping of epigenetic changes is essential for understanding the mechanisms involved in chemical-induced gene regulation. The identification of DNA methylation patterns is a common procedure in the study of epigenetics, as methylation is known to have significant effects on gene expression, and is involved with normal development as well as disease. Thus, the ability to discriminate between methylated DNA and non-methylated DNA is essential for generating methylation profiles for such studies. Methylated DNA immunoprecipitation (MeDIP) is a recently devised method for the extraction of methylated DNA from a sample of interest and used to determine the distribution of DNA-methylation within functional regions (e.g., promoters) or in the entire genome robustly and cost-efficiently. This approach is based on the enrichment of methylated DNA with an antibody that specifically binds to 5-methyl-cytosine (5mC) and can be combined with PCR and microarray. Here we summarize the experimental procedure of MeDIP followed by high throughput microarray and provides a comprehensive summary of quality control strategies.
URI
http://pubs.kist.re.kr/handle/201004/40769
ISSN
2005-9752
Appears in Collections:
KIST Publication > Article
Files in This Item:
There are no files associated with this item.
Export
RIS (EndNote)
XLS (Excel)
XML


qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

BROWSE