Gold nanoparticles surface-functionalized with paclitaxel drug and biotin receptor as theranostic agents for cancer therapy
- Gold nanoparticles surface-functionalized with paclitaxel drug and biotin receptor as theranostic agents for cancer therapy
- 허동녕; 양대혁; 문호진; 이정복; 배민수; 이상천; 이원준; 선인철; 권일근
- Gold nanoparticles; Paclitaxel; Biotin; Beta-cyclodextrin; Theranostic agents
- Issue Date
- VOL 33, 856-866
- We describe in this study whether the gold nanoparticle (AuNP) surface-functionalized with PEG, biotin,
paclitaxel (PTX) and rhodamine B linked beta-cyclodextrin (β-CD) (AuNP-5´) can be useful as a theranostic
agent for cancer therapy without the cytotoxic effect on normal cells. Prior to surfacefunctionalizing
AuNPs, the cytotoxicity of the nanoparticles was evaluated, followed by their cytocompatibility.
PTX, an anti-cancer agent, formed inclusion complexations with β-CD conjugated AuNPs,
and effectively released from the AuNP-2´ surface-functionalized with PEG, beta-cyclodextrin (β-CD) and
paclitaxel (PTX) using the intracellular glutathione (GSH) level (10 mM). Two types of AuNP-4 surfacefunctionalized
with PEG and rhodamine B linked β-CD and AuNP-5 surface-functionalized PEG, biotin
and rhodamine B linked β-CD were used for evaluating their specific interaction on cancer cells such as
HeLa, A549 and MG63. These were also tested against normal NIH3T3 cell, determining that the AuNP-5
was more effectively involved with the cancer cells. Confocal laser scanning microscopy (CLSM),
fluorescence-activated cell-sorting (FACS) and cell viability analyses showed that the AuNP-5´ plays
a significant role in the diagnosis and therapy of the cancer cells, and may be used in theranostic agents.
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