Peptide receptor-based selective dinitrotoluene detection using a microcantilever sensor
- Peptide receptor-based selective dinitrotoluene detection using a microcantilever sensor
- 황교선; 이민혁; 이주희; 여운석; 이정훈; 김강민; 강지윤; 김태송
- Microcantilever; Resonant frequency; Dinitrotoluene; Electronic nose; Artificial olfactory system
- Issue Date
- Biosensors and bioelectronics
- VOL 30, NO 1, 249-254
- We reported that peptide could be utilized as receptor molecule in the gas phase for application in
micro/nano sensors by using a specific peptide that recognizes 2,4-dinitrotoluene at room temperature
and in an atmospheric environment and measuring changes in the resonant frequency of the peptide
immobilized microcantilevers. By using these peptides as receptors on a microcantilever sensor, we were
able to experimentally detect 2,4-dinitrotoluene (DNT) vapor at concentrations as low as parts per billion
(ppb) in the gas phase. While resonant frequency changes after binding between 2,4-DNT and the specific
peptide receptor that was immobilized on microcantilevers were observed, the resonant frequency of
DNT nonspecific peptide immobilized microcantilever did not change when exposed to 2,4-DNT vapor.
The limit of detection (LOD) was calculated to be 431 ppt of limit of detection is numerically expected
by experimental based on an equation that describes the relationship between the noise-equivalent
analyte concentration. These results indicate that the peptide receptors hold great promise for use in
the development of an artificial olfactory system and electronic nose based on micro/nanotechnology
for monitoring various chemical vapors in the gas phase such as explosive mixtures of chemicals and/or
volatile organic compounds.
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