Cyclic ADP Ribose-Dependent Ca2+ Release by Group I Metabotropic Glutamate Receptors in Acutely Dissociated Rat Hippocampal Neurons
- Cyclic ADP Ribose-Dependent Ca2+ Release by Group I Metabotropic Glutamate Receptors in Acutely Dissociated Rat Hippocampal Neurons
- 손종우; 유연진; 이도연; 신희섭; 이호석; 호원경
- Issue Date
- PLOS ONE
- VOL 6, NO 10, e26625-1-e26625-8
- Group I metabotropic glutamate receptors (group I mGluRs; mGluR1 and mGluR5) exert diverse effects on neuronal and
synaptic functions, many of which are regulated by intracellular Ca2+. In this study, we characterized the cellular
mechanisms underlying Ca2+ mobilization induced by (RS)-3,5-dihydroxyphenylglycine (DHPG; a specific group I mGluR
agonist) in the somata of acutely dissociated rat hippocampal neurons using microfluorometry. We found that DHPG
activates mGluR5 to mobilize intracellular Ca2+ from ryanodine-sensitive stores via cyclic adenosine diphosphate ribose
(cADPR), while the PLC/IP3 signaling pathway was not involved in Ca2+ mobilization. The application of glutamate, which
depolarized the membrane potential by 28.5±4.9 mV (n = 4), led to transient Ca2+ mobilization by mGluR5 and Ca2+ influx
through L-type Ca2+ channels. We found no evidence that mGluR5-mediated Ca2+ release and Ca2+ influx through L-type
Ca2+ channels interact to generate supralinear Ca2+ transients. Our study provides novel insights into the mechanisms of
intracellular Ca2+ mobilization by mGluR5 in the somata of hippocampal neurons.
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