The Rapid Identification and Preparative Isolation of Antioxidant Compounds using On-line HPLC-ABTS+ Detection and Centrifugal Partition Chromatography
- The Rapid Identification and Preparative Isolation of Antioxidant Compounds using On-line HPLC-ABTS+ Detection and Centrifugal Partition Chromatography
- 이희주; 전제승; 메즈박; 김철영
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- High-performance liquid chromatography coupled to an on-line ABTS+-based assay (on-line HPLC-ABTS+) system was developed to identify the principle antioxidants in azalea flowers and red sanders. Three major flavonoids, myricetin, quercetin and kaempferol, recovered in ethyl acetate extracts of azalea flowers were determined to have antioxidant activities. In red sanders, five major flavonoids showed antioxidant activities in on-line HPLC-ABTS+ system. These antioxidant flavonoids were isolated and purified by centrifugal partition chromatography (CPC).
Preparative purification of azalea flowers extract was performed with two successive different biphasic solvent systems, consisting of n-hexane-ethyl acetate-methanol-water (3:5:3:5 or 4:5:4:5, v/v), and n-hexane-ethyl acetate-methanol-water (4:6:4:6, v/v) was used to purify the antioxidant flavonoids in ethyl acetate extracts of red sanders. The structures were identified by their retention time, UV spectra, ESI-MS and NMR.
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