The Rapid Identification of Antioxidants of Salsola komarovii Extract by the HPLC coupled to an on-line ABTS+ based assay and HPLC-ESI/MS

Title
The Rapid Identification of Antioxidants of Salsola komarovii Extract by the HPLC coupled to an on-line ABTS+ based assay and HPLC-ESI/MS
Authors
이희주판철호김철영
Issue Date
2011-10
Publisher
2011 International Symposium and Annual Meeting of the KSABC
Abstract
Online HPLC-ABTS+ based assay system and HPLC-ESI/MS were developed to rapidly determine and identify the phenolic antioxidants in Salsola komarovii extracts. The extracts was separated by HPLC and the elute was mixed with a stabilized solution of ABTS+ radical scavenging detection system. The negative peaks, which indicated the antioxidant activity, were monitored by measuring the decrease in absorbance at 734 nm. HPLC-ESI/MS were used to identify the seven flavonoids and two phenolic amides in S. komarovii extracts. The antioxidant compounds were separated and identified as rutin, isoquercitrin, astragalin, and isorhametin.(SRAA)
URI
http://pubs.kist.re.kr/handle/201004/41270
Appears in Collections:
KIST Publication > Conference Paper
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