Disulfide-crosslinked heparin-pluronic nanogels as a redox-sensitive nanocarrier for intracellular protein delivery
- Disulfide-crosslinked heparin-pluronic nanogels as a redox-sensitive nanocarrier for intracellular protein delivery
- D. H. Nguyen; 최종훈; 정윤기; 박기동
- nanogels; heparin; pluronic; protein; redox-sensitive nanocarrier; glutathione; RNase A; heparin-Pluronic nanogels; intracellular protein delivery
- Issue Date
- Journal of bioactive and compatible polymers
- VOL 26, NO 3, 287-300
- Improving the efficacy of drug delivery via nanocarriers has been a major issue in the field of
intravenous delivery. In this study, a polymeric nanogel was developed to enhance the stability,
redox responsiveness, and the efficacy for intracellular protein delivery. The thiolated heparin-
Pluronic conjugate was self-assembled and oxidized to form a disulfide-crosslinked nanogel
network under a diluted aqueous condition. The disulfide-crosslinked heparin-Pluronic (DHP)
nanogels with encapsulated RNase A were characterized by in vitro release and cytotoxicity tests
depending on the existence of glutathione (GSH). The DHP nanogels exhibited reduced
hydrodynamic size, higher encapsulation degree, and augmentable release responding to the
GSH concentration. The ctotoxicity data confirmed that DHP nanogels were more effective
for the intracellular delivery of RNase A compared to non-crosslinked nanogel.
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