RGD-Conjugated In Situ Forming Hydrogels as Cell-Adhesive Injectable Scaffolds

Title
RGD-Conjugated In Situ Forming Hydrogels as Cell-Adhesive Injectable Scaffolds
Authors
N Q. Tran정윤기이유진박경민박기동
Keywords
hydrogel; RGD peptide; injectable scaffold; in situ forming hydrogels; enzymatic reaction; cyclodextrin; chitosan; horse radish peroxidase (HRP)
Issue Date
2011-03
Publisher
Macromolecular research
Citation
VOL 19, NO 3, 300-306
Abstract
In this study, a bioactive hydrogel was prepared from a chitosan derivative and Arg-Gly-Asp (RGD)-conjugated polypseudorotaxane, which is a cell-adhesive extracellular matrix. Chitosan was modified with 4-hydroxy phenyl acetic acid to obtain a water-soluble product for enzymatic cross-linking. Tyramine-terminated polypseudorotaxane (PRx) was prepared from the inclusion complex of a tyramine-terminated poly(ethylene glycol) backbone, and α-cyclodextrin (α-CD). Gly-Arg-Gly-Asp-Ser (GRGDS) was conjugated to the PRx using 4-nitrophenyl chloroformate (NPC) and partially carboxylated with succinic anhydride. The structure of the PRx-RGD and 4-hydroxylphenylacetamide chitosan (CHPA) was characterized by 1H NMR and FTIR spectroscopy. The RGD content in PRx-RGD was determined to be 0.19%. PRx-RGD and CHPA solution was crosslinked to form a bioactive hydrogel in the presence of horseradish peroxidase (HRP) and hydrogen peroxide (H2O2), which exhibited rapid gelation (~20 sec). An in vitro cell culture was carried out with L929 mouse fibroblasts for 1 and 3 days. The results showed that fibroblasts adhered better and appeared to be more biocompatible on the RGD-conjugated hydrogel than the hydrogel without RGD. The combined results highlight the potential use of this bioactive hydrogel as an injectable scaffold in tissue engineering applications.
URI
http://pubs.kist.re.kr/handle/201004/41365
ISSN
1598-5032
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KIST Publication > Article
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