Effect of Agarase Signal Peptide from Agarivorans albus YKW-34 on Protein Secretion in Escherichia coli

Title
Effect of Agarase Signal Peptide from Agarivorans albus YKW-34 on Protein Secretion in Escherichia coli
Authors
이주영송대근손진기판철호
Keywords
Agarivorans albus; Escherichia coli; recombinant protein; secretion; signal peptide
Issue Date
2010-06
Publisher
Journal of applied biological chemistry.
Citation
VOL 53, NO 2, 105-107
Abstract
To overcome the limitation of E. coli expression system such as inclusion body formation and disulfide bond failure, we tried to express the heterologous protein as a secreted form. We adopted agarase signal peptide (ASP; 23 amino acid residues) from Agarivorans albus YKW-34 which is one of marine bacteia. When we used ASP to express β-agarase, about 42% activity was detected in media.
URI
http://pubs.kist.re.kr/handle/201004/41728
ISSN
1976-0442
Appears in Collections:
KIST Publication > Article
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